anti cd36  (Bioss)

Journal: Frontiers in Pharmacology

Article Title: α-Mangostin prevents diabetic cardiomyopathy by inhibiting oxidative damage and lipotoxicity through the AKT–FOXO1–CD36 pathway

doi: 10.3389/fphar.2025.1566311

Figure Lengend Snippet: Primers for RT-qPCR.

Article Snippet: These included BCL-2 (68103-1), ACTB (81115-1-RR), Nrf2 (16396-1-AP), CPT1β (22170-1), ACADM (55210-1), and PPARα (66826-1), all purchased from Proteintech (Wuhan, China); BAX (#2772), SOD2 (#13141), p-FOXO1 (#9461), FOXO1 (#2880), and caspase9 (#9508), purchased from Cell Signaling Technology (Inc., MA, United States); cleaved-caspase 3 (WL01992), cleaved-caspase 9 (WL01838), and HO-1 (WL02400), purchased from Wanleibio (Shenyang, China); CD36 (bs-8873R) and p-AKT (bs-0876R), purchased from Bioss (Beijing, China); and AKT (179463, Abcam).

Techniques: Sequencing

Journal: Frontiers in Pharmacology

Article Title: α-Mangostin prevents diabetic cardiomyopathy by inhibiting oxidative damage and lipotoxicity through the AKT–FOXO1–CD36 pathway

doi: 10.3389/fphar.2025.1566311

Figure Lengend Snippet: A-MG reduces lipid accumulation in HG/F-induced H9C2 cells. (A) Heatmap depicting A-MG-induced changes in gene expression influencing fat digestion and absorption. (B) GSEA gene enrichment map. (C) RT-qPCR analysis of CD36, PPARα, and CPT1β mRNA levels in H9C2 cells. (D) Representative images of intracellular lipid droplets in H9C2 cells stained with BODIPY 493/503. (E) Quantification of the mean fluorescence intensity of BODIPY 493/503; IntDen/Area (A.U.), integrated density/area (arbitrary unit). (F–H) Western blot analysis of p-FOXO1, FOXO1, and CD36 protein expressions in H9C2 cells. (I) Representative images of immunofluorescent staining of FOXO1 in H9C2 cells. (J) Quantification of the FOXO1 immunofluorescence signal in H9C2 cells. (K–M) Analysis of docking between A-MG and CD36 proteins. (K) 3D structure of CD36 bound with A-MG; the structure of CD36 is shown in cyan and A-MG is rendered in green. (L) Depiction of the electrostatic surface of the A-MG binding site on CD36. (M) Detail of the binding mode of A-MG on CD36. The results represent at least three independent experiments; data are expressed as the mean ± SD (n ≥ 3). * p < 0.05, ** p < 0.01, and *** p < 0.001.

Article Snippet: These included BCL-2 (68103-1), ACTB (81115-1-RR), Nrf2 (16396-1-AP), CPT1β (22170-1), ACADM (55210-1), and PPARα (66826-1), all purchased from Proteintech (Wuhan, China); BAX (#2772), SOD2 (#13141), p-FOXO1 (#9461), FOXO1 (#2880), and caspase9 (#9508), purchased from Cell Signaling Technology (Inc., MA, United States); cleaved-caspase 3 (WL01992), cleaved-caspase 9 (WL01838), and HO-1 (WL02400), purchased from Wanleibio (Shenyang, China); CD36 (bs-8873R) and p-AKT (bs-0876R), purchased from Bioss (Beijing, China); and AKT (179463, Abcam).

Techniques: Gene Expression, Quantitative RT-PCR, Staining, Fluorescence, Western Blot, Immunofluorescence, Binding Assay

Journal: Frontiers in Pharmacology

Article Title: α-Mangostin prevents diabetic cardiomyopathy by inhibiting oxidative damage and lipotoxicity through the AKT–FOXO1–CD36 pathway

doi: 10.3389/fphar.2025.1566311

Figure Lengend Snippet: A-MG activates the AKT pathway. (A–E) Western blot analysis examining the effect of LY294002 on AKT, p-AKT, Nrf2, SOD2, and HO-1 protein expression in HG/F-induced H9C2 cells. (F–I) Western blot analysis examining the effect of LY294002 on FOXO1, p-FOXO1, CD36, and CPT1β expression in HG/F-induced H9C2 cells. The results represent at least three independent experiments; data are expressed as the mean ± SD (n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

Article Snippet: These included BCL-2 (68103-1), ACTB (81115-1-RR), Nrf2 (16396-1-AP), CPT1β (22170-1), ACADM (55210-1), and PPARα (66826-1), all purchased from Proteintech (Wuhan, China); BAX (#2772), SOD2 (#13141), p-FOXO1 (#9461), FOXO1 (#2880), and caspase9 (#9508), purchased from Cell Signaling Technology (Inc., MA, United States); cleaved-caspase 3 (WL01992), cleaved-caspase 9 (WL01838), and HO-1 (WL02400), purchased from Wanleibio (Shenyang, China); CD36 (bs-8873R) and p-AKT (bs-0876R), purchased from Bioss (Beijing, China); and AKT (179463, Abcam).

Techniques: Western Blot, Expressing

Journal: Frontiers in Pharmacology

Article Title: α-Mangostin prevents diabetic cardiomyopathy by inhibiting oxidative damage and lipotoxicity through the AKT–FOXO1–CD36 pathway

doi: 10.3389/fphar.2025.1566311

Figure Lengend Snippet: A-MG attenuates lipid accumulation and oxidative stress in the cardiac tissue of DCM mice. (A) Representative images of myocardial BODIPY 493/503 staining. (B–D) Analysis of serum TG, TC, and LDL-C levels. (E–M) Western blot analysis of p-AKT, AKT, p-FOXO1, FOXO1, CD36, PPARα, CPT1β, Nrf2, HO-1, and SOD2 in heart tissues. Data are presented as the mean ± SD. In (B–D) , n = 5; in (F, H–M) , n = 4; in (G) , n = 3. * p < 0.05, ** p < 0.01, and *** p < 0.001.

Article Snippet: These included BCL-2 (68103-1), ACTB (81115-1-RR), Nrf2 (16396-1-AP), CPT1β (22170-1), ACADM (55210-1), and PPARα (66826-1), all purchased from Proteintech (Wuhan, China); BAX (#2772), SOD2 (#13141), p-FOXO1 (#9461), FOXO1 (#2880), and caspase9 (#9508), purchased from Cell Signaling Technology (Inc., MA, United States); cleaved-caspase 3 (WL01992), cleaved-caspase 9 (WL01838), and HO-1 (WL02400), purchased from Wanleibio (Shenyang, China); CD36 (bs-8873R) and p-AKT (bs-0876R), purchased from Bioss (Beijing, China); and AKT (179463, Abcam).

Techniques: Staining, Western Blot